| 出品公司: | ATCC |
|---|---|
| 菌种名称: | ATCC TIB-71, ATCC TIB-71 |
| 菌株类型: | RAW264.7 |
| 存储人: | WC Raschke |
| 单元格类型: | 巨噬细胞；转化Abelson小鼠白血病病毒 |
| 产品目录号: | TIB-71 |
| 低温保存: |
冷冻培养基：添加5%（v/v）二甲基亚砜的完整生长培养基
储存温度：液氮汽相
|
| 生长条件: |
大气：空气，95%；二氧化碳（CO2），5%
温度：37°C
|
| 生物安全等级: | 2 |
| 应用: |
该细胞系是合适的转染宿主。
细胞将胞饮中性红，吞噬乳胶珠和酵母多糖。它们能够对绵羊红细胞和肿瘤细胞靶点进行抗体依赖性裂解。LPS或PPD治疗2天刺激红细胞溶解，但不刺激肿瘤细胞靶点。
|
| 菌株特点: |
ATCC TIB-71 |
| 参考文献: |
Ralph P, Nakoinz I. Antibody-dependent killing of erythrocyte and tumor targets by macrophage-related cell lines: enhancement by PPD and LPS. J. Immunol. 119: 950-954, 1977. PubMed: 894031
Raschke WC, et al. Functional macrophage cell lines transformed by Abelson leukemia virus. Cell 15: 261-267, 1978. PubMed: 212198
Denlinger LC, et al. Regulation of inducible nitric oxide synthase expression by macrophage purinoreceptors and calcium. J. Biol. Chem. 271: 337-342, 1996. PubMed: 8550583
Hambleton J, et al. Activation of c-Jun N-terminal kinase in bacterial lipopolysaccharide-stimulated macrophages. Proc. Natl. Acad. Sci. USA 93: 2774-2778, 1996. PubMed: 8610116
Taylor GA, et al. Identification of anovel GTPase, the inducibly expresed GTPase, that accumulates in response to interferon gamma. J. Biol. Chem. 271: 20399-20405, 1996. PubMed: 8702776
Li YM, et al. Molecular identity and cellular distribution of advanced glycation endproduct receptors: relationship of p60 to OST-48 and p90 to 80K-H membrane proteins. Proc. Natl. Acad. Sci. USA 93: 11047-11052, 1996. PubMed: 8855306
Panneerselvam K, Freeze HH. Mannose enters mammalian cells using a specific transporter that is insensitive to glucose. J. Biol. Chem. 271: 9417-9421, 1996. PubMed: 8621609
Lokuta MA, et al. Mechanisms of murine RANTES chemokine gene induction by newcatle disease virus. J. Biol. Chem. 271: 13731-13738, 1996. PubMed: 8662857
Taylor MF, et al. In vitro efficacy of morpholino-modified antisense oligomers directed against tumor necrosis factor-alpha mRNA. J. Biol. Chem. 271: 17445-17452, 1996. PubMed: 8663413
Standard Practice for Testing for Biological Responses to Particles in Vitro. West Conshohocken, PA:ASTM International;ASTM Standard Test Method F 1903-98R03.
Hartley JW, et al. Expression of infectious murine leukemia viruses by RAW264.7 cells, a potential complication for studies with a widely used mouse macrophage cell line. Retrovirology. 4: 5:1, 2008. PubMed 18177500.
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